Limabean Agar (Fava-Feijao) - 500G

Lima bean agar é um meio de ágar que é usado para cultivar os esporos de Phytophthora sojae. Phytophthora sojae causa a ferrugem da soja. A ferrugem da soja pode afetar seriamente a produção e a qualidade da soja.

é recomendado para cultivo de fungos fitopatológicos e outros. Ã‰ composto por uma infusão de feijão seco e ágar. A infusão de Limabean fornece todos os nutrientes essenciais para o crescimento dos fungos. O pH do meio é 5,6, o que permite um crescimento exuberante de fungos.

Limabean Agar is recommended for cultivation of phytopathological and other fungi. Composition** Ingredients Gms / Litre Lima beans, infusion (from 62.5g) 08.000 Agar 15.000 Final pH ( at 25°C) 5.6±0.2 **Formula adjusted, standardized to suit performance parameters Directions Suspend 23 grams in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Mix well and pour into sterile Petri plates. Principle And Interpretation Plant diseases are caused by a variety of living organisms (called pathogens) such as fungi, bacteria, viruses, nematodes, phytoplasmas, protozoa, and parasitic plants, and by non-living agents such as air pollutants, nutrient imbalances, and various environmental factors. Fungi are responsible for many diseases of plants, causing large losses in crop production. They are responsible for contaminating harvested crops with mycotoxins dangerous for public health. As such crops are the target of large-scale fungicide use, with the corresponding environmental and health hazards. Limabean Agar is recommended for cultivation of such phytopathological and other fungi (1). Limabean Agar is composed of an infusion of dry lima beans and agar. Limabean infusion provides all essential growth nutrients for fungi. The pH of the medium is 5.6, which enables luxuriant fungal growth. Quality Control Appearance Cream to yellow homogeneous free flowing powder Gelling Firm,comparable with 1.5% Agar gel Colour and Clarity of prepared medium Yellow coloured opalescent gel forms in Petri plates and may have slight precipitate. Reaction Reaction of 2.3% w/v aqueous solution at 25°C. pH : 5.6±0.2 pH 5.40-5.80 Cultural Response M736: Cultural characteristics observed after an incubation at 28-32°C for 40-48 hours. Organism Growth *Aspergillus brasiliensis ATCC 16404 luxuriant Candida albicans ATCC 10231 luxuriant Saccharomyces cerevisiae ATCC 9763 luxuriant Storage and Shelf Life Store below 30°C in tightly closed container and the prepared medium at 2-8°C. Use before expiry date on the label. Reference 1. Atlas R. M., 2004, Handbook of Microbiological Media, Lawrence C. Parks (Ed.), 3rd Edition, CRC Press.